Table 1.
Transcript abundance of MEP, MVA and other terpenoid backbone biosynthesis pathway genes as per the S. guaranitica transcriptome data annotation
| Pathway | Gene name | Kegg entry | Gene bank accession ID | EC.No. | Read in leaf | FPKM |
|---|---|---|---|---|---|---|
| MEP | SgDXS 1 | K01662 | KX869088 | 2.2.1.7 | 8968.47 | 223.94 |
| SgDXS2 | K01662 | KX869089 | 2.2.1.7 | 40 | 41.89 | |
| SgDXS3 | K01662 | KX869090 | 2.2.1.7 | 169 | 3.68 | |
| SgDXS4 | K01662 | KX869091 | 2.2.1.7 | 3634.82 | 85.89 | |
| SgDXS5 | K01662 | KX869092 | 2.2.1.7 | 697.93 | 14.94 | |
| SgDXR 1 | K00099 | KX869093 | 1.1.1.267 | 4080.05 | 175.32 | |
| SgDXR 2 | K00099 | KX869094 | 1.1.1.267 | 158.7 | 37.97 | |
| SgMCT | K00991 | KX869095 | 2.7.7.60 | 563.54 | 25.17 | |
| SgCMK | K00919 | KX869096 | 2.7.1.148 | 1588.67 | 53.65 | |
| SgHDS 1 | K03526 | KX869097 | 1.17.7.1 | 350 | 13.57 | |
| SgHDS2 | K03526 | KX869098 | 1.17.7.1 | 123.02 | 8.35 | |
| SgHDS3 | K03526 | KX869099 | 1.17.7.1 | 11 | 4.08 | |
| SgHDS4 | K03526 | KX869100 | 1.17.7.1 | 17316.85 | 304.95 | |
| SgHDR 1 | K03527 | KX869101 | 1.17.1.2 | 4 | 2.72 | |
| SgHDR2 | K03527 | KX869102 | 1.17.1.2 | 850 | 175.65 | |
| SgHDR3 | K03527 | KX869103 | 1.17.1.2 | 5 | 2.7 | |
| SgHDR4 | K03527 | KX869104 | 1.17.1.2 | 1034 | 209.85 | |
| SgHDR5 | K03527 | KX869105 | 1.17.1.2 | 296 | 16.78 | |
| SgHDR6 | K03527 | KX869106 | 1.17.1.2 | 858.92 | 106.05 | |
| SgHDR7 | K03527 | KX869107 | 1.17.1.2 | 67 | 17.91 | |
| SgHDR8 | K03527 | KX869108 | 1.17.1.2 | 9 | 4.12 | |
| SgHDR9 | K03527 | KX869109 | 1.17.1.2 | 42686.56 | 1228.23 | |
| SgHDR10 | K03527 | KX869110 | 1.17.1.2 | 43 | 2.94 | |
| SgIDI1 | K01823 | KX869111 | 5.3.3.2 | 2 | 1.27 | |
| SgIDI2 | K01823 | KX869112 | 5.3.3.2 | 2344.77 | 98.18 | |
| SgIDI3 | K01823 | KX869113 | 5.3.3.2 | 1 | 1.44 | |
| MVA | SgAACT 1 | K00626 | KX869114 | 2.3.1.9 | 624.59 | 22.69 |
| SgAACT 2 | K00626 | KX869115 | 2.3.1.9 | 2001.02 | 67.38 | |
| SgHMGS | K01641 | KX869116 | 2.3.3.10 | 1897.92 | 61.34 | |
| SgHMGR1 | K00021 | KX869117 | 1.1.1.34 | 40 | 20.67 | |
| SgHMGR2 | K00021 | KX869118 | 1.1.1.34 | 2300.82 | 56.02 | |
| SgHMGR3 | K00021 | KX869119 | 1.1.1.34 | 23 | 4.09 | |
| SgHMGR4 | K00021 | KX869120 | 1.1.1.34 | 70 | 11.16 | |
| SgHMGR5 | K00021 | KX869121 | 1.1.1.34 | 144 | 25.27 | |
| SgHMGR6 | K00021 | KX869122 | 1.1.1.34 | 1691.49 | 68.13 | |
| SgHMGR7 | K00021 | KX869123 | 1.1.1.34 | 14 | 2.17 | |
| SgHMGR8 | K00021 | KX869124 | 1.1.1.34 | 39 | 20.82 | |
| SgHMGR9 | K00021 | KX869125 | 1.1.1.34 | 14 | 1.25 | |
| SgMVK1 | K00869 | KX893913 | 2.7.1.36 | 441.39 | 15.27 | |
| SgMVK2 | K00869 | KX893914 | 2.7.1.36 | 4 | 1.83 | |
| SgPMK | K00938 | KX893915 | 2.7.4.2 | 754 | 19.93 | |
| SgMDC | K01597 | KX893916 | 4.1.1.33 | 786.17 | 56.1 | |
| Monoterpene | SgGPPS | K14066 | KX893917 | 2.5.1.1 | 1292.8 | 47.81 |
| Sesqui and triterpene | SgFPPS | K00787 | KX893918 | 2.5.1.10 | 1909.92 | 80.21 |
| Diterpene | SgGGPSΙΙ1 | K13789 | KX893925 | 2.5.1.29 | 1393 | 60.95 |
| SgGGPSΙΙ2 | K13789 | KX893926 | 2.5.1.29 | 2153.95 | 74.07 | |
| SgGGPSΙΙ3 | K13789 | KX893919 | 2.5.1.29 | 2 | 0.67 | |
| SgGGPSΙΙ4 | K13789 | KX893920 | 2.5.1.29 | 125 | 9.99 | |
| SgGGPSΙΙ5 | K13789 | KX893921 | 2.5.1.29 | 54.01 | 25.45 | |
| SgGGPSΙΙ6 | K13789 | KX893927 | 2.5.1.29 | 180.01 | 12.7 | |
| SgGGPSΙΙ7 | K13789 | KX893922 | 2.5.1.29 | 25 | 5.21 | |
| SgGGPSΙΙ8 | K13789 | KX893923 | 2.5.1.29 | 44 | 6.97 | |
| SgGGPSΙΙ9 | K13789 | KX893924 | 2.5.1.29 | 16 | 7.69 | |
| SgGGPSΙΙ10 | K13789 | KX893928 | 2.5.1.29 | 673.01 | 28.66 | |
| Other terpenoid backbone biosynthesis | SgFOHSDR | K15890 | KX893929 | 1.1.1.216 | 241.13 | 9.68 |
| SgFOLK1 | K15892 | KX893930 | 2.7.1. | 998.85 | 85.9 | |
| SgFOLK2 | K15892 | KX893931 | 2.7.1. | 892.1 | 43.74 | |
| SgPCYOX1 | K05906 | KX893932 | 1.8.3.5 1.8.3.6 | 523.75 | 14.1 | |
| SgSTE24 | K06013 | KX893933 | 3.4.24.84 | 1467.53 | 48.98 | |
| SgCHLP1 | K10960 | KX893934 | 1.3.1.83 | 14506.79 | 419.11 | |
| SgCHLP2 | K10960 | KX893935 | 1.3.1.83 | 83 | 10.71 | |
| SgCHLP3 | K10960 | KX893936 | 1.3.1.83 | 675.74 | 26.22 | |
| SgFACE2 | K08658 | KX893937 | 3.4.22.- | 297 | 36.52 | |
| SgPCME1 | K15889 | KX893938 | 3.1.1.- | 232.82 | 11.54 | |
| SgPCME2 | K15889 | KX893939 | 3.1.1.- | 36 | 1.81 | |
| SgFNTB | K05954 | KX893940 | 2.5.1.58 | 626.09 | 21.79 | |
| SgSPS | K05356 | KX893941 | 2.5.1.84 2.5.1.85 | 6500.62 | 224.55 | |
| SgDHDDS1 | K11778 | KX893942 | 2.5.1.87 | 129.01 | 7.82 | |
| SgDHDDS2 | K11778 | KX893943 | 2.5.1.87 | 4818.35 | 227.35 | |
| SgDHDDS3 | K11778 | KX893944 | 2.5.1.87 | 778.03 | 31.45 | |
| SgDHDDS4 | K11778 | KX893945 | 2.5.1.87 | 3175 | 179.52 | |
| SgDHDDS5 | K11778 | KX893946 | 2.5.1.87 | 219 | 9.45 | |
| SgICMT1 | K00587 | KX893947 | 2.1.1.100 | 187 | 15.99 | |
| SgICMT2 | K00587 | KX893948 | 2.1.1.100 | 119 | 12.97 |
FPKM, fragments per kilobase of transcripts per million mapped fragments; DXS, 1-deoxy-d-xylulose-5-phosphate synthase; DXR, 1-deoxy-d-xylulose-5-phosphate reductoisomerase; MCT, 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase; CMK, 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase; HDS, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase; HDR, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase; IDI, isopentenyl-diphosphate delta-isomerase, AACT, acetyl-CoA C-acetyltransferase; HMGS, hydroxylmethylglutaryl-CoA synthase; HMGR, hydroxymethylglutaryl-CoA reductase (NADPH); MVK, mevalonate kinase; PMK, 5-phosphomevalonate kinase, MDC, mevalonate diphosphate decarboxylase; GPPS, geranyl diphosphate synthase; FPPS, farnesyl pyrophosphate synthase; GGPS, geranylgeranyldiphosphate synthase, type II; FOHSDR, farnesol dehydrogenase; FOLK, farnesol kinase; PCYOX1, prenylcysteine oxidases/farnesylcysteine lyase; STE24, STE24 endopeptidases; CHLP, geranylgeranyl diphosphate reductase; FACE2, farnesylated protein-converting enzyme 2; PCME, prenylcysteine alpha-carboxyl methylesterase; FNTB, protein farnesyltransferase subunit beta; SPS, all-trans-nonaprenyl-diphosphate synthase; DHDDS, ditrans, polycis-polyprenyl diphosphate synthase; ICMT, protein-S-isoprenylcysteine O-methyltransferase.