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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cell Signal. 2018 Sep 20;53:68–79. doi: 10.1016/j.cellsig.2018.09.012

Fig. 2. hsBAFF downregulates the protein levels of LC3-II with a concomitant increase of cell proliferation and viability in B cells.

Fig. 2

Raji cells and purified mouse splenic B lymphocytes were stimulated with hsBAFF (0–5 μg/ml) for 12 h and 48 h, respectively, or with 2.5 μg/ml hsBAFF for indicated time. (A and B) Total cell lysates were subjected to Western blotting using indicated antibodies. The blots were probed for β-actin as a loading control. Similar results were observed in at least three independent experiments. (C and D) The cell proliferation was evaluated by cell counting. (E and F) The cell viability was determined by the MTS assay. All quantified data were expressed as mean ± SEM (n = 5). Using one-way ANOVA, *P < 0.05, **P < 0.01, difference vs 0 μg/ml hsBAFF group or 0 h group.