Skip to main content
. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Leukemia. 2018 Jul 2;32(12):2710–2714. doi: 10.1038/s41375-018-0190-1

Figure 1. Diplotyping of NUDT15 variants and tolerated 6-MP dose.

Figure 1.

(A) Major haplotypes of NUDT15. (B) Diplotype analysis by droplet digital PCR. Variant-specific probes (FAM for c.36_37insGGAGTC and HEX for c.415C>T) were able to distinguish compound heterozygous variants (*3/*6) and mono-allelic variants (*1/*2). (C) Diplotype analysis by restriction enzyme-PCR. They were cleaved with HpyCH4III and PCR was performed to confirm the results of ddPCR. (D) Average 6MP doses according to diplotype. Three cases without a NUDT15 variant (*1/*1) had a TPMT heterozygous variant (c.719A>G), shown in red triangles.