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. Author manuscript; available in PMC: 2020 Jan 1.

Published in final edited form as: J Steroid Biochem Mol Biol. 2018 Aug 14;185:110–117. doi: 10.1016/j.jsbmb.2018.08.004

Table 2.

Kinetic parameters of zfSts determined at different reaction temperatures^a

	at 28°C			at 37°C

	V_max (pmol/min/mg)	K_m (μM)	V_max/K_m	V_max (pmol/min/mg)	K_m (μM)	V_max/K_m
Cho-sulfate	81.25 ± 2.4	0.62 ± 0.06	131.7	141.8 ± 6.7	0.96 ± 0.13	148.0
DHEA-sulfate	374.3 ± 25.4	19.38 ± 3.07	19.3	453.8 ± 22.6	15.72 ± 1.98	28.9
E1-sulfate	1262.0 ± 60.8	4.32 ± 0.49	292.3	1766.0 ± 35.2	3.39 ± 0.18	521.4
E2-sulfate	1070.0 ± 44.7	2.07 ± 0.29	516.9	1647.0 ± 73.8	1.93 ± 0.30	855.6
Preg-sulfate	104.1 ± 1.2	0.18 ± 0.01	571.7	185.4 ± 3.2	0.25 ± 0.01	741.6

^a

Results represent means ± SD derived from three determinations.

^b

Kinetic parameters were determined based on the equation for Michaelis-Menten kinetics.