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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Plant J. 2018 Oct 23;96(6):1106–1120. doi: 10.1111/tpj.14091

Figure 1. NADPH oxidase dependent O2- is generated during ER Stress partially through RBOHD and RBOHF activity.

Figure 1.

In situ detection and semi-quantification of superoxide in root tissues by staining with nitro-tetrazolium blue (NBT) A. WT or rbohd rbohf seedlings treated with 1.0 μg/ml Tm, 1.0 μg/ml TM + 2.5 μM DPI, or DMSO were stained with NBT after 24 or 48 hours. †: mature zone; ‡: maturation zone; Δ: root tip. B. Tissue-specific differences in superoxide are shown by relative pixel intensities of NBT stain from the root tip ± SE.