Time course gene expression analysis of WT, rbohd rbohf, ire1a ire1b, and bzip60 bzip28 seedlings subjected to ER stress. qRT-PCR analyses were performed using primers specific for either RBOHF (A), RBOHD (B), ZAT12 (C), spliced bZIP60 (sbZIP60; D), BIP3 (E), or ERdj3B (E). Data represent the mean ratio ± SE (biological replicates=3); Statistical significance compared to equivalent WT value, unless a bracket is used to indicate comparison. Statistical significance determined using Student’s unpaired t-test and indicated by: *=p<0.05, **=p<0.005 ***=p<0.005, NS=not significant.