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. 2018 Dec 5;9:3024. doi: 10.3389/fmicb.2018.03024

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Copyright © 2018 Xin, Guo, Mu and Kong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metabolic pathways associated with acetoin biosynthesis in L. casei. (A) The metabolic engineering strategies for aerobic acetoin overproduction. The dashed arrows indicated that the pathways were disrupted. ldh, encoding Lactate dehydrogenase; als, encoding α-acetolactate synthase; aldB, encoding acetolactate decarboxylase; pdhC, encoding pyruvate dehydrogenase complex E2 component; pflB, encoding pyruvate formate lyase; adhE, encoding alcohol dehydrogenase; pta: encoding phosphate acetyltransferase; ackA: encoding acetate kinase. PTS, phosphotransferase system. (B) One colony of the BL27 strain was selected to be tested by PCR with the forward primer of the up homology and the reverse primer of the down homology after Cre recombinase induction and incubation. The expected PCR fragment from the deletion type is about 2.0 kb, while the wild-type is approximately 5.0 kb for pflB and 3.0 kb for hicD3, ldh, pdhC.