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. 2018 Dec 5;9:3024. doi: 10.3389/fmicb.2018.03024

Table 1.

Plasmids and bacterial strains used in this study.

Strain or plasmid Characteristic(s) Source
Strains
Escherichia coli
DH5α Subcloning host Novagen
XL1-Blue Subcloning host Novagen
Lactobacillus casei
BL23 Wild-type Maze et al., 2010
BL24 Derivative of L. casei BL23 (ΔhicD3) This study
BL25 Derivative of L. casei BL23 (ΔhicD3, ΔpflB) This study
BL26 Derivative of L. casei BL23 (ΔhicD3, ΔpflB, Δldh2) This study
BL27 Derivative of L. casei BL23 (ΔhicD3, ΔpflB, Δldh2, ΔpdhC) This study
Plasmids
pUC19 Ampr; cloning vector Novagen
pET-28a Kanr; cloning vector Novagen
pMSP3535 Ermr Bryan et al., 2000
pMSPcre Source of cre gene Xin et al., 2017
pUCgalK Source of fragment loxP-cat-loxP Xin et al., 2017
pMSP456-Cre Expression LCABL_13040-50-60 under PnisA control and Cre under Plac control This study