Table 1.
Strain or plasmid | Characteristic(s) | Source |
---|---|---|
Strains | ||
Escherichia coli | ||
DH5α | Subcloning host | Novagen |
XL1-Blue | Subcloning host | Novagen |
Lactobacillus casei | ||
BL23 | Wild-type | Maze et al., 2010 |
BL24 | Derivative of L. casei BL23 (ΔhicD3) | This study |
BL25 | Derivative of L. casei BL23 (ΔhicD3, ΔpflB) | This study |
BL26 | Derivative of L. casei BL23 (ΔhicD3, ΔpflB, Δldh2) | This study |
BL27 | Derivative of L. casei BL23 (ΔhicD3, ΔpflB, Δldh2, ΔpdhC) | This study |
Plasmids | ||
pUC19 | Ampr; cloning vector | Novagen |
pET-28a | Kanr; cloning vector | Novagen |
pMSP3535 | Ermr | Bryan et al., 2000 |
pMSPcre | Source of cre gene | Xin et al., 2017 |
pUCgalK | Source of fragment loxP-cat-loxP | Xin et al., 2017 |
pMSP456-Cre | Expression LCABL_13040-50-60 under PnisA control and Cre under Plac control | This study |