Table 2.
Oligonucleotide primers used in this study.
| Primer | Sequence (5′-3′) | Restriction site |
|---|---|---|
| PlF | TATCTCGAGTAGCACTGATCATTAAAGAAC | XhoI |
| PlR | ACGGTCAGTAAATTGGACATGTTGTCATCACCTCCCAGTG | |
| creF | ACATGTCCAATTTACTGACCGTAC | |
| creR | TTAATCGCCATCTTCCAGCA | |
| 456F | AACTGCAGATGACCATGCTTGATTACAACACAG | PstI |
| 456R | TGCTGGAAGATGGCGATTAGCTACTCGACTAGCTCATCCATGCT | |
| catF | GAAAGATCTTACCGTTCGTATAATGTATGC | BglII |
| catR | GAAAGATCTTACCGTTCGTATAGCATACAT | BglII |
| catF1 | CCGCTCGAGTACCGTTCGTATAATGTATGC | XhoI |
| catR1 | CCGCTCGAGTACCGTTCGTATAGCATACAT | XhoI |
| catF2 | ATCTGCAGTACCGTTCGTATAATGTATGC | PstI |
| catR2 | ATCTGCAGTACCGTTCGTATAGCATACAT | PstI |
| hicD3-uF | AATGAATTCTGTTACGCAGAATGTTGACGG | EcoRI |
| hicD3-uR | GATCTAGAATAAAAAATCTCCTTTTCAAAATGC | XbaI |
| hicD3-dR | TATAAGCTTAGCGCATCATGGTTAAATCG | HindIII |
| hicD3-dF | ATAAAGCTTAAGTAGGTCCTTTTACGAGCG | HindIII |
| pflB-uF | GAACTCGAGTTTCTCGTGATGGCTACGTTAA | XhoI |
| pflB-uR | AGTTGTTTGCCTCCTAAAGTGG | |
| pflB-dR | CAAAAGCTTGTGTCTTTGAGGAAAAAATGCG | HindIII |
| pflB-dF | CCACTTTAGGAGGCAAACAACTAGATCTGCAGCTTAACTCAAGACAGGAA | BglII |
| ldh-uF | AATGAATTCTCGACAAAACTCATCGCTGC | EcoRI |
| ldh-uR | GTGATATCATCCTTTCTTATGTGC | |
| ldh-dF | GCACATAAGAAAGGATGATATCACCCTCGAGGACATCGAAACTCGTCAGTA | XhoI |
| ldh-dR | ATCAT GATCTAGAGTGACATAATTTGTAATGGCTTGG | XbaI |
| pdhC-uF | ACATGTCGACTTCCTAAAGGACACGTACGA | SalI |
| pdhC-uR | GTTGGCACCTCTTCTTTCTAG | |
| pdhC-dF | CTAGAAAGAAGAGGTGCCAACCTGCAGGCATGGTTGTAGGCGATTT | PstI |
| pdhC-dR | ACATGCATGCATCCCAGTTGTTGCAAGTTC | SphI |
The restriction sites in the primer sequences are underlined.