Table 3.

Experimental details for the single-plasmid system.

Strains designation	Target gene	Donor strains	Length of the deleted region (bp)	Efficiencya
				Recombineering	Marker excision
BL24	hicD3	BL23	906	2/2	6/10
BL25	pflB	BL24	2262	2/2	6/10
BL26	ldh	BL25	981	2/2	4/10
BL27	pdhC	BL26	1665	2/2	6/10

^a“m/n” indicated that “n” colonies were randomly selected for screening and “m” colonies were demonstrated to be correct.