FIGURE 3.

Details of the perinuclear association and envelopment of multiple neuronal nuclei by LPS in sporadic Alzheimer’s disease (AD) brain (Brodmann Area 22; superior temporal lobe neocortex); (A–C) details of LPS association with neuronal nuclei in three AD independent brain samples; all three AD brain samples were from moderate-to-advanced-AD (CDR 2.0-3.0); no such extensive association between LPS and control brain nuclei was observed (Figure 1); lipopolysaccharide (LPS; red stain; λmax = 690 nm); DAPI (nuclear-specific blue stain; λmax = 470 nm); NeuN (neuron-specific green stain; λmax = 520 nm); brain sections are 10 μm thick (see text); a large number of neuronal nuclei in sporadic AD were found to be completely enveloped by LPS especially in the later stages of sporadic AD (CDR 3.0); scale bar for all photos here (lower right) = 20 μm.