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. 2018 Dec 11;9(12):1187. doi: 10.1038/s41419-018-1244-z

Fig. 4. Decreased GPx1 expression induces autophagy.

Fig. 4

a GPx1, LC3, and β-actin expression levels were measured by immunoblot analysis in PDA cells following the silencing of GPx1 in DMEM and were compared with those in the control cells. b Expression levels of LC3, SQSTM1 and β-actin in GPx1-silenced cells in DMEM were examined by western blot analysis following treatment with CQ (10 μM) for 24 h. c GPx1-silenced cells in DMEM were treated with Rap (100 nM) for 24 h, and the indicated protein levels were then analyzed by western blotting. d MiaPaCa-2 cells in DMEM were transfected with GFP-mRFP-LC3B, treated with CQ (10 μM) or Rap (100 nM) for 24 h, and then observed using a confocal microscope to assess changes in green and red fluorescence (Scale bar: 20μm). The number of acidified autophagosomes (GFP−RFP+) versus the number of neutral autophagosomes (GFP+RFP+) per cell in each condition are quantified in e (n.s., no significance, *P < 0.05, **P < 0.01)