Fig. 10.

Assessment of anti-Candida mAbs in murine models of disseminated candidiasis and a routine diagnostic assay. a C. albicans SC5314 was pre-incubated with saline, IgG1 control, anti-whole cell mAb (AB119) or anti-Hyr1 mAb (AB120) and then injected i.v. into the tail vein of female BALB/c mice (n = 6 per group). Kidney fungal burdens from each group were determined on day 3 post infection. b IgG1 control or anti-whole cell mAb (AB119) was administered i.p. 4 h prior to injection of C. albicans SC5314 i.v. into the lateral tail vein of male CD1 mice (n = 10 per group). Kidney fungal burdens from each group were determined on day 7 post infection. Dots represent individual animals and horizontal lines represent mean; statistical significance was determined by two-tailed t-test; *P < 0.05, **P < 0.01. Purified anti-Candida mAbs react with yeast (c) and hyphal (d) antigenic preparations in a counterimmunoelectrophoresis assay routinely employed to diagnose Candida infection. Precipitin lines between wells indicate a positive reaction