Fig. 3.

Visualising anti-Candida mAbs binding to C. albicans cells. a Representative immunofluorescent images from three separate experiments with anti-Hyr1 mAb AB120 against WT CAI4-CIp10, Hyr1 null mutant and a Hyr1 reintegrant strain. b Also shown are immunofluorescent images and corresponding immunogold localisation of anti-whole cell mAbs binding to C. albicans yeast and hyphal cell walls. A fluorescently conjugated secondary goat anti-human IgG antibody was used to detect anti-Candida mAb binding. Fluorescent images are representative images from three separate experiments. Transmission electron microscopy (TEM) images show representative cells from at least one experiment. Scale bars represent 4 µm on immunofluorescent images and 100 nm on TEM images