Fig. 5.

Characterization of potential DUO1 target genes in Marchantia. a RNA in situ hybridization of genes involved in sperm morphogenesis, the ortholog of AtDAZ1 and AtDAZ2 genes, and RWP-RK transcription factors during spermatogenesis (see Supplementary Figure 2 for sense-probe controls). Bars, 25 μm. Cell shape schematics (top) represent the developmental stages referred to in Fig. 1c. b Expression levels of genes involved in sperm morphogenesis, the ortholog of AtDUO1 target genes, and RWP-RK transcription factors in antheridiophores of WT (gray bars) and Mpduo1-1^ko (white bars). The expression of each gene in WT is set to 1. Error bars indicate mean ± SD; n = 3 (*p < 0.05; **p < 0.01; ns, not significant; Student’s t-test)