FIGURE 5.

Studies with the recombinant VZV/34.5 virus. (A) Immunolabeling of the HSV1 ICP34.5 protein after infection with rVZV/34.5. The ICP34.5 protein is labeled green. (B) Control immunolabeling with the anti-ICP34.5 antibody in wild-type VZV infection. No ICP34.5 protein was detected. (C) Small focus of VZV/34.5 infection in SOC at 14 dpi. Larger infectious foci were first seen at 28 dpi. (D) Graph of autophagosome counts for VZV-32 and VZV/34.5 strains in SOC at 28 dpi. Six individual sections were examined. There was no statistical difference. (E–H) Imaris renderings of confocal micrographs in SOC infected with VZV/34.5 for 28 days. VZV-induced syncytia were apparent in each panel because VZV gE immunolabeling was detectable around multiple nuclei without evidence of any barriers by individual cellular membranes. VZV glycoprotein gE is labeled red; autophagic puncta are labeled green; nuclei are labeled blue.