Figure 10.

Expression of selected muscle differentiation markers and β-catenin target genes in ß-cat^fl/flPtch^del/+Myf5^CreER/wt animals. (A) H&E staining of a representative RMS and corresponding skeletal muscle (SM) of the same mouse to check tissue for tumor or skeletal muscle components before RNA isolation. (B) qRT-PCR of muscle differentiation markers and β-catenin target genes of RMS from untreated (n = 4), vehicle- (n = 5), or TAM-treated mice (n = 5). All data were normalized to GAPDH and are shown as fold change expression to the expression of the same gene within skeletal muscle (SM) of the respective mouse, which was set to 1. Bars show the mean and SEM of one cohort and represent measurements in technical duplicates. One-Way ANOVA with Dunn's test for multiple comparisons show no significant differences. Tam: tamoxifen-treated at an age of 4 weeks. Vehicle: vehicle-treated at an age of 4 weeks.