Fig. 4.

Tissue-specific depletion of CSF1R in RP-Mps and their progenitors results in loss of DCs. a Scheme of the generation of Rank-iCre mice (iCre = codon-improved Cre). The iCre-recombinase was knocked into exon 1 of the Rank gene. b Immunohistology on spleen sections from 3-week-old Rank-iCre⁺;Td-rfp⁺ mice, ×40 objective was used for picture acquisition, scale bar corresponds to 20 μm. c Lineage tracing of RANK-expressing cells in adult Rank-iCre⁺;eYFP^ki/wt mice. LT-HSC long-term HSCs, MPP multipotent progenitors, MP myeloid progenitors, LC Langerhans cells. SD is shown. Gatings are shown in Supplementary Fig. 1a and Supplementary Fig. 3b-d. d Dot plots show the labeling of fetal liver Kit⁺ Sca-1⁺ Lineage⁻ (KSL) hematopoietic stem and progenitor cells (top) and yolk sac CD11b⁺ F4/80^hi macrophages (bottom) from E18.5 Rank-iCre⁺;eYFP^ki/wt embryos. e Lineage tracing in Rank-iCre;eYFP^ki/wt embryos gating on CD11b^lo F4/80^hi embryonic macrophages in the yolk sac at the indicated time points. SD is shown. f Plots show the frequencies (left, second right) and fold changes (second left, right) of CD11b^lo F4/80^hi RP-Mps or CD11b⁺ F4/80^lo macrophages and spleen leukocytes from newborn Rank-iCre⁺;Csf1r^F/− and control mice. Two-sided t test (left and second right) and Mann–Whitney U test (right and second right) were performed. SD is shown. g Plots show frequencies and fold changes of DCs and spleen leukocytes from Rank-iCre⁺;Flt3^−/−;Csf1r^F/− and control mice at 3 weeks of age. Two-sided t test (left) and Mann–Whitney U test (right) were performed. SD is shown