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. 2018 Dec 11;9:5279. doi: 10.1038/s41467-018-07685-x

Fig. 5.

Fig. 5

Depletion of spleen CD11blo F4/80hi macrophages in adult FLT3-deficient mice results in loss of DCs. a Schematic outline of the experiment. b Clodronate liposomes (Clod) were injected into Flt3−/− and wild-type (WT) mice and the efficient depletion of blood monocytes (CD11b+ Gr-1lo) was analyzed 24 h later (left). c Plot shows the reduction of blood monocytes. Data are pooled from two independent experiments. SD is shown. d Cell numbers of spleen macrophages (left), RP-Mps (middle), and DCs (right) normalized to non-injected controls from the same experiments 1–2 weeks after Clod injection. All cell types were depleted efficiently. Data are pooled from two independent experiments. SD is shown. e Cell numbers of spleen macrophages (left), RP-Mps (middle), and DCs (right) normalized to non-injected controls from the same experiments 4 weeks after Clod injection. Macrophages recovered in all mice. RP-Mps failed to recover in all mice. DCs recovered in Flt3-proficient but not in Flt3-deficient mice. Two-sided t tests were performed and SD is shown throughout the figure. SD is shown