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. 2018 Dec 11;9:5281. doi: 10.1038/s41467-018-07671-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — AbCYP82M3 catalyzes the synthesis of tropinone from 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoic acid. Extracted ion LC/MS chromatograms in positive-ion mode (m/z 140.108 ± 0.02) for the tropinone (7) [M + H]⁺ ion from an authentic standard and a representative AbCYP82M3 enzyme reaction. AbCYP82M3 enzyme reactions lacking either NADPH or 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoic acid (5) as well as those performed using microsomes extracted from the untransformed BY4742 yeast strain are included as negative controls