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Kinetic properties of AbPYKS
| Variable substrate (concentration in assay) | Co-substrate (concentration in assay) | K m | V max | K i | k cat | kcat/Km |
|---|---|---|---|---|---|---|
| (µM) | (nmol s–1 mg–1) | (µM) | (s–1) | (mM–1 s–1) | ||
| N-methyl-Δ1-pyrrolinium (0.0031–0.4 mM) | Malonyl-CoA (0.1 mM) | 10 ± 0.6a | 283 ± 22 | 103 ± 16 | 12.3 ± 1.0 | 1174 ± 52 |
| Malonyl-CoA (0.0063–0.8 mM) | N-methyl-Δ1-pyrrolinium (0.05 mM) | 12 ± 1 | 230 ± 3 | 280 ± 0.9 | 10 ± 0.1 | 848 ± 90 |
a±Error values represent the standard error of the mean for three replicate assays
