Fig. 2.

Leukaemia latencies differ with different ages of cells of origin. a Graph of percentage of cells that homed to BM, PB and spleen 18 h post transplant of NH9 LSK^FL (n = 3), LSK^3w (n = 3), LSK^10w (n = 3) and LSK^>52w (n = 3). Significance determined by Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. b Percentage of GFP⁺ cells in PB at 16w. Graph depicts mean + s.d. from two independent experiments. Significance determined by one-way ANOVA and Bonferroni post test (p = 0.44). c Kaplan–Meier survival curve of recipients transplanted with NH9 transduced LSKs from P2. Recipients censored at death. Significance determined by overall log rank, *p < 0.05. d Kaplan–Meier survival curve of recipients pooled into young (NH9^FL and NH9^3W) and adult (NH9^10W and NH9^>52W). Recipients censored at death. Significance determined by overall log rank, **p < 0.01. e Leukaemic burden assessed by BM and spleen cellularity, and spleen weight. f Analysis of PB parameters of recipients with donor derived leukaemia. e, f Graphs show individual results with mean ± s.d. Red dotted lines depict normal range. Young n = 11, adult n = 15. ns not significant as determined by Student’s t-test. g Assessment of leukaemic infiltration as %GFP in BM, spleen and PB after recipient mice developed donor derived leukaemia, from young and adult groups. Young n = 10, adult n = 15. Graphs depict mean + s.d. ns not significant as determined by Student’s t-test. h Graph of the total number of mice from two independent experiments developing donor-derived leukaemia in young and adult groups. i Expression of immature surface markers on GFP⁺ cells from BM of recipients that succumbed to NH9 AML. Graphs show individual result with mean ± s.d. Young n = 8, adult n = 15. ns not significant as determined by Student’s t-test. All data representative of two independent experiments. NH9^FL n = 10, NH9^3w n = 10, NH9^10w n = 5, NH9^>52w n = 10, young n = 20 and adult n = 15 unless otherwise stated