Fig. 3.

Different leukaemia lineage phenotypes due to age of cell of origin. a Summary for the outcome for young or adult mice developing NH9⁺ leukaemia. Average expression of CD11b, CD11b/Gr-1, CD19 and B220 in GFP⁺ BM cells is shown with expression range. For IHC ‘−‘ denotes neoplastic cells are negative for the marker, ‘+’ denotes 20%, ‘++’ 20–40%, ‘+++’ 40–60% and ‘++++’ more than 60% positive expression in neoplastic cells. b Bar chart of lineage of all donor derived leukaemia showing data pooled into young (NH9^FL and NH9^3w) (n = 10) and adult (NH9^10w and NH9^>52w) (n = 15). Graphs depict mean + s.d. from two independent experiments. Significance determined by two-way ANOVA, *p < 0.05. c Representative flow cytometry plots from each disease phenotype AML (top row), MPAL (middle) and ALL (bottom). Flow cytometry plots depict CD11b, Gr-1, B220, CD19, CD8 and CD4 expression through GFP in the BM. d Representative ×40 and ×60 IHC images of sternal sections from each disease phenotype AML (top row), MPAL (middle) and ALL (bottom). Left two columns shows MPO and right 2 columns shows CD3. Positive cells exhibit brown cytoplasmic staining. Black scale bar represents 20 µm for ×40 images and 500 µm for ×60 images. Relative gene expression of e myeloid f B-lymphoid and g T-lymphoid genes in AML (n = 3), MPAL (n = 4) and ALL (n = 2) generated in vivo from young LSKs transformed with NH9. Graphs depict mean LFC + s.d