Fig. 3.

RNAi phenotyping for potential candidates. RNAi was performed using two pyrethroid resistant colonies, Tiassalé and VK7. 3–5 day old females mosquitoes were injected with dsRNA from each of the respective transcripts. 72-h post injection, the mosquitoes were exposed to 0.05% WHO deltamethrin papers for 1 h and mortality scored 24 h later. a Mortality associated with dsRNA induced knockdown in Tiassalé mosquitoes. b dsRNAs showing significantly increased mortality in Tiassalé mosquitoes were then injected into a second resistant colony, VK7. c Knockdown levels for each dsRNA construct relative to GFP-injected controls (Tiassalé population only). Error bars represent standard deviation, three biological replicates and three technical replicates were used for each gene with each circle representing the mean value for each biological replicate in the qPCR data and significance is indicated by *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, as computed by ANOVA with post hoc Tukey correction. Numbers on the bars represent number of mosquitoes tested under each condition