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Electrophoretic Mobility Shift assays. GST fused protein of PdWND1B binds to the promoters of (A) PdHB3 and (B) Myb002 and IQD10. GST fused PdHB3 protein binds to the promoters of (D) PdIQD10 and (E) CaM014 but not to (C) Actin, suggesting a gene regulatory network downstream of PdWND1B. Promoter regions corresponding to the 250 bp upstream of the transcriptional start site of the respective genes were used for biotin labeling and shift experiments.
