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Proliferation of WT, miR-146a KO, and miR-146b KO B cells in response to LPS, anti-CD40, and anti-IgM antibody. Splenic B cells (CD19+) were incubated for 48 hours in the presence of media only (none) or the indicated mitogens (0.1, 1, or 10 μg/mL LPS; 0.1, 1, or 5 μg/mL anti-CD40; and 10 μg/mL anti-IgM). Proliferation was measured by [3H]-thymidine incorporation. Data are presented as mean ± SEM. n = 6. *P < .05, **P < .01. cpm, counts per minute.
