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. 2018 Dec 5;2(23):3447–3461. doi: 10.1182/bloodadvances.2018022053

Figure 4.

Figure 4.

Osteogenic and adipogenic differentiation of MSCs is modulated by AZA in healthy MSCs. (A) Experimental design. Healthy and MDS MSCs were treated with 10 µM AZA on days 0 and 7. Untreated MSCs were used as controls. MSCs were differentiated for 18 days and oil-red-o staining plus Harris-hematoxylin or alizarin-red staining were used to quantify adipogenesis (C) or osteogenesis (B), respectively. Differentiation was ranked according to absent (=0), weak (=1), moderate (=2), or intense (=3) staining. (B-C) Representative images (top panel) and statistical summary (bottom panel) for (B) mean osteogenic potential of n = 5 healthy and n = 5 MDS MSCs ± SEM and (C) mean adipogenic potential of n = 10 healthy and n = 7 MDS MSCs ± SEM. *P < .05; **P < .01; ***P < .001.