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Effect of polyamines on polypeptide synthesis in cell-free systems (A) and stimulation of the assembly of 30S ribosomal subunits (B). A, poly(U)-directed (panel a), Qβ RNA-directed (panel b), and T7 DNA (panel c)-directed protein synthesis was measured by changing Mg2+ concentrations. PUT, putrescine; SPD, spermidine. B, methylation of adenines at 1,518 and 1,519 in 16S rRNA on 23S core particles was stimulated by polyamines.
