Figure 8.

miR-451a levels in sera were correlated with splenic CD11c⁺ cells in vivo. A–C, schematic of preparation and measurement of samples (A). Mouse sera were collected from C57BL/6 mice. miR-21 and miR-451a levels in serum EVs were determined and were normalized to miR-16 levels. Splenic CD11c⁺ cells were isolated from C57BL/6 mice, and intracellular miR-21 and miR-451a levels were determined. The correlation between miRNA levels in serum EVs and in splenic CD11c⁺ cells was calculated, and statistical analyses were performed (p < 0.05, r represents Pearson correlation coefficient) (B and C). The data are representative of at least two independent experiments. D and E, schematic of treatment and preparation of the samples (D). Sera were collected from C57BL/6 mice, and miR-451a levels in serum EVs were determined. 25 μg of inactivated WV (50 μl volume) was intranasally administered into those mice. 6 h after administration, sera were collected. IP-10 levels in sera were determined by ELISA. The correlation between miRNA levels in serum EVs and in splenic CD11c⁺ cells was calculated, and statistical analyses were performed (p < 0.05, r represents Pearson correlation coefficient) (E). The data are a representative of two independent experiments.