Figure 12.

Effect of phosphorylation at Ser-13 and/or Ser-16 on the subcellular localization of mutant (Q₄₂) PFFs in striatal neurons. A, immunostaining using the MW8 antibody revealing nuclear localization of internalized pSer-13/pSer-16-Q₂₂ PFFs in a proportion of neurons. A low-magnification view is shown in the left panel, and the right panel shows high-resolution images of an individual neuron exhibiting nuclear localization. B, significantly more neurons treated with phosphorylated Q₂₂ and Q₄₂ Httex1 PFFs exhibit nuclear localization per 0.1 mm² compared with the controls treated with unmodified Q₂₂ or Q₄₂ Httex1 PFFs. The asterisks denote p < 0.001 determined by one-way analysis of variance and Scheffé post hoc statistical analysis. Error bars, S.D.