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. 2018 Oct 12;293(48):18636–18645. doi: 10.1074/jbc.RA118.005190

Figure 2.

Figure 2.

nadB is required for normal CCV formation. Shown are representative micrographs (three independent experiments) of HeLa cells infected for 3 days with C. burnetii NMII, the nadB::Tn 23-H5 mutant, the nadB::Tn (23-H5) complemented strain, the nadB::Tn 7-G9 mutant, and the nadB::Tn (7-G9) complemented strain. The cells were stained with anti-Coxiella antibody (red), anti-LAMP1 antibody (green), and DAPI (blue), and white asterisks indicate representative CCVs. Note that the CCVs containing the nadB mutant appear smaller and contain few bacteria compared with either the WT NMII WT strain or the complemented strains. The scale bars represent 10 μm.