Figure 5.

Key amino acid residues are conserved between E. coli NadB and Coxiella NadB. Protein sequence alignment was performed using Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/; Ref. 33). (Please note that the JBC is not responsible for the long-term archiving and maintenance of this site or any other third party hosted site.) The conserved amino acids were predicted to be involved substrate binding (His²⁴⁴, His³³⁶, and Arg³⁷¹, highlighted green), and the catalytic site (Arg²⁷⁵, highlighted blue) was identified in Coxiella NadB. Numbers on the right side indicate position of amino acids for both organisms. Conserved regions are indicated by black shading and asterisks; regions of similarity are indicated by colons.