Table 1.

Primers used in this study

Name	Sequence (5′ to 3′)	Annealing temperature (°C)	Purpose
nadB F1	AAAGTCGACATGCCCACCTATGATGTCCT	62	Complementation construct
nadB F2	AAAGTCGACATATGCCCACCTATGATGTCCT	61	Cloning into pGEX-4T-1
nadB R	AAAGTCGACCTAAAATTGCTTTTCGCCGATT	62	Cloning into pGEX-4T-1 or complementation construct
nadB (Internal)	CGTCTGTAAGGGCGCAGAGG	55	Sequencing within the inserted region
OmpA0008 F	CAGAGCCGGGAGTCAAGCT	55	Quantification of Coxiella genomes
OmpA0009 R	CTGAGTAGGAGATTTGAATCGC	55	Quantification of Coxiella genomes
pKM225 (5172) F	TGCTCACATGTTCTTTCCTGC	55	Transposon detection
pKM225 (5173) R	TGTGATGGCTTCCATGTCG	55	Transposon detection
pJB-Kan:3xFLAG F	GAGCTGTTGACAATTAATCATC	55	Sequencing complementation construct
pJB-Kan:3xFLAG R	GGATTCATCGACTGTGGCCG	55	Sequencing complementation construct
T7	TAATACGACTCACTA	55	Sequencing
SP6	TATTTAGGTGACACT	55	Sequencing
pGEX-4T-1 F	GGGCTGGCAAGCCACGTTTGGTG	55	Sequencing pGEX-nadB construct
pGEX-4T-1 R	CCGGGAGCTGCATGTGTCAGAGG	55	Sequencing pGEX-nadB construct
R275L F	GGAGATGGCCCCTCTAGACATTGTCGCTC	55	Site-directed mutagenesis
R275L R	GAGCGACAATGTCTAGAGGGGCCATCTCC	55	Site-directed mutagenesis