Figure 4.

Growth behavior of S. cerevisiae WT strain BY4741 expressing different Lr34 versions. A, cells were either transformed with an empty vector (EV) or plasmids for the expression of Lr34sus, inactive Lr34sus (Lr34susW), Lr34res, or inactive Lr34res (Lr34resW) and grown in selective SD media under standard growth conditions. A₆₀₀ of freshly inoculated overnight grown cultures (starting A₆₀₀ = 0.1) was measured at the indicated time points during the exponential growth phase. Values are means ± S.E. (n ≥5). B, immunoblot analysis of microsomal yeast fractions expressing different HA-tagged versions of Lr34.