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. 2018 Dec 5;9:2847. doi: 10.3389/fimmu.2018.02847

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Copyright © 2018 Liu, Feng, Zhu, Huang, Chen, Pan, June, Zheng and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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U0126-EtOH decrease the proliferating cells induced by SHH signaling. (A) Cells of the blank control group. (B) Cells were treated with vehicle (DMSO in DMEM supplemented with 10% FBS). (C) Cells were treated with Purmorphamine (1 μM). (D) Cells were co-treated with U0126-EtOH (10 μM) and Purmorphamine(1 μM). (E) Cells were treated with Cyclopamine (10 μM). The proliferating cells were detected by the flow cytometry and the ratio of EdU positive cells were calculated (F). The results represent the mean ± SD based on ≥3 replicates. ^*P < 0.05 vs. control group. ^**P < 0.01 vs. control group.