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. 2018 Dec 5;9:1797. doi: 10.3389/fpls.2018.01797

FIGURE 4.

FIGURE 4

Decreased tolerance of Ubi:OsDIRP1-sGFP transgenic rice plants in response to salt stress. (A) The salt stress phenotypes of wild-type and T4 Ubi:OsDIRP1-sGFP plants. Light-grown, 5-week-old wild-type and T4 Ubi:OsDIRP1-sGFP (lines #1, #2, and #3) plants were treated with 200 mM NaCl for 16–18 days and then transferred to normal growth conditions with watering for 1 month. OE represents OsDIRP1-overexpressing transgenic rice plants. (B) Survival rates of wild-type (WT) and T4 Ubi:OsDIRP1-sGFP plants in response to salt stress. Data are means ± SE (n ≥ 5 independent biological experiments; >40 plants were used in each assay, ∗P < 0.05, ∗∗P < 0.01, Student’s t-test). (C) Leaf disk senescence assays in response to high salinity. Leaf disks (0.5 cm in diameter) were prepared from 5-week-old wild-type and transgenic plants and floated in different concentrations (0, 200, 400, 600, and 800 mM) of NaCl for 3 days. A representative photo was taken after 3 days of incubation. (D) Chlorophyll content in the leaf disk senescence assay. The amounts of chlorophyll (chlorophyll a + chlorophyll b) in the wild-type and T4 Ubi:OsDIRP1-sGFP (lines #1, #2, and #3) leaf disks were determined 3 days after incubation with different concentrations (0, 200, 400, 600, and 800 mM) of NaCl. Data are means ± SE (n ≥ 4 independent biological experiments, ∗∗P < 0.01, Student’s t-test).