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. 2018 Dec 5;9:1797. doi: 10.3389/fpls.2018.01797

FIGURE 6.

FIGURE 6

Increased tolerance of Ubi:OsDIRP1-sGFP transgenic rice plants to cold stress. (A) Cold stress phenotypes of wild-type and T4 Ubi:OsDIRP1-sGFP transgenic plants. Light-grown, 5-week-old wild-type and T4 Ubi:OsDIRP1-sGFP (lines #1, #2, and #3) plants were transferred to a cold room at 4°C for 8 days, after which the plants were recovered at 28°C for 27 days. OE represents OsDIRP1-overexpressing transgenic plants. (B) Survival rates of wild-type (WT) and T4 Ubi:OsDIRP1-sGFP plants in response to cold stress. Data are means ± SE (n ≥ 6 independent biological experiments; >30 plants were used in each assay, ∗∗P < 0.01, Student’s t-test). (C) Electrolyte leakage analysis of wild-type (WT) and T4 Ubi:OsDIRP1-sGFP plants in response to cold stress. Electrolyte leakage analysis was conducted using 8-day-old wild-type and transgenic seedlings at different time points before and after cold (4°C) treatment (0, 5, and 10 days). Data are means ± SD (n = 3 independent biological experiments; 12 plants of each genotype were used in each experiment, ∗P < 0.05, ∗∗P < 0.01, Student’s t-test). (D) Total leaf chlorophyll content of wild-type and T4 Ubi:OsDIRP1-sGFP (lines #1, #2, and #3) transgenic rice plants before and after cold treatment. The amounts of leaf chlorophyll (chlorophyll a + chlorophyll b) of mock-treated (before cold) and cold-treated plants were determined 1 month after recovery from cold stress. Data are means ± SE (n ≥ 3 biological independent experiments; 30 plants were used in each assay, ∗∗P < 0.01, Student’s t-test).