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. 2018 Dec 5;9:1797. doi: 10.3389/fpls.2018.01797

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Copyright © 2018 Cui, Min, Byun, Oh and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of cold stress-inducible genes in wild-type and OsDIRP1-overexpressing transgenic rice plants. Light-grown, 10-day-old wild-type and Ubi:OsDIRP1-sGFP transgenic plants were exposed to cold (4°C) stress for 0 or 24 h. The induction patterns of five stress-responsive genes, OsDREB1A, OsDREB1B, OsDREB1D, GAD, and MRP4, were analyzed by real-time qRT-PCR. The relative expression of each gene was normalized to that of OsActin. Data are means ± SE (^∗P < 0.05, ^∗∗P < 0.01, Student’s t-test) of three independent experiments.