FIG 1.

DDX3 regulates the expression of PACT, STAT1, GNB2, Rac1, TAK1, and p38 MAPK proteins in THP-1 and HeLa cells. THP-1 and HeLa cells were transduced with either the empty lentiviral vector (pLKO.1) or the pLKO.1 vector expressing the indicated shRNAs. After 24 h, puromycin was added to the culture medium for selection. The cells were harvested for analysis at 3 days posttransduction. Immunoblotting was performed using antibodies against DDX3, α-tubulin, PACT, STAT1, GNB2, Rac1, TAK1, and p38 MAPK. The detection of α-tubulin served as a loading control.