FIG 7.

Recruitment of phagocytes to inflammation sites is impaired in DDX3a/b knockdown zebrafish. Control MO- and DDX3a/b MO-injected transgenic zebrafish (mpx:EGFP) larvae at the long-pec stage (2 days postfertilization) were anesthetized using 4.2% tricaine, and tail fin injury was inflicted using a sterile scalpel to induce inflammation (top). Each zebrafish larva was placed in a layer of 3% (wt/vol) methylcellulose in a 35-mm glass-bottom dish. Live imaging showed the recruitment of neutrophils at the indicated time points after tail fin transection (middle). The number of recruited neutrophils was determined by sampling 4 or 5 zebrafish larvae per group (bottom). Data are shown as means ± SDs from four independent experiments. *, P < 0.05; **, P < 0.01.