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. 2018 Dec 11;18:203. doi: 10.1186/s12935-018-0700-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Double immunofluorescence (DIF) staining with confocal microscopy to evaluate the co-expression of IL-33 with its receptor, ST2, in the ESCC section. DIF images showed that IL-33-IR (labelled by Texas red, red cells in A, D) was frequently co-localized (merged images in C, F) with ST2-IR (labelled by FITC, green cells in B, E) in ESCC cells (merged image in C) and stromal cells (merged image in F). IRs for targeted proteins were not shown in isotopy-matched negative controls (G–I). (A–I: DIFs, original magnification ×200; counterstaining was not applied)