Fig. 4.

Validation of RNA sequencing results by RT-qPCR. a Correlations between gene expression levels measured by RT-qPCR and RNA-Seq methods. b Comparison of RNA-Seq log₂FoldChange read counts with log₂FoldChange RT-qPCR copy numbers. The upper panel shows the RNA-Seq read counts (log₂FoldChange) for seven genes, of which five are upregulated in chronic hypoxia vs. normoxia in L. mandarinus and L. brandtii brain and two are downregulated. The lower panel shows log₂FoldChange by RT-qPCR copy numbers for the same genes in chronic hypoxia vs. normoxia in L. mandarinus and L. brandtii brain samples. (LM: L. mandarinus; LB: L. brandtii)