Table 1.
Bacterial strains and oligonucleotide primers used in the present study.
| Strain/Mutant | Relevant Genotype, Phenotype or Sequence | Reference |
|---|---|---|
| P. chlororaphis PA23 | RifR; WT [soybean root tip isolate] PHZ+PRN+ | [2] |
| PA23-63 | PHZ- RifR phzE::Tn5-OT182 genomic fusion | [12] |
| PA23-8 | PRN- RifR prnBC deletion mutant | [12] |
| PA23-63-1 | PHZ- PRN- RifR phzE::Tn5-OT182 genomic fusion; prnBC deletion mutant | [12] |
| PA23rpoS | PA23 with pKNOCK inserted into rpoS | [25] |
| PA23gacS | PHZ- RifR gacS::Tn5-OT182 genomic fusion | [3] |
| PA23relA/spoT | PA23 with pKNOCK-Gm inserted into relA; TetR cassette inserted into spoT | [25] |
| PA23rpoS | PA23 with pKNOCK-Tc inserted into rpoS | [13] |
| Primers | ||
| phzA-FOR | 5′-GACTGGCAATGGCACAAC-3′ | [14] |
| phzA-REV | 5′-GCAATAACCTTCGGGATAACC-3′ | [14] |
| phzI-FOR | 5′-CGATGCCGTTGTTCTGG-3′ | [14] |
| phzI-REV | 5′-AGCCGTTCGTAGTGGACTC-3′ | [14] |
| phaF-FOR | 5′-GAAAAAGAAGGCAGCTCGTG-3′ | This study |
| phaF-REV | 5′-ATCGACTTTCTTGCCGACAG-3′ | This study |
| phaI-FOR | 5′-CTACACCAAGGTCGGTCAGG-3′ | This study |
| phaI-REV | 5′-ATCCAGCTGCACTTCGACTT-3′ | This study |
| phaD-FOR | 5′-CTGGGTATCGCTGACCAGTT-3′ | This study |
| phaD-REV | 5′-ACTACCGCTTCCTGTTCCAG-3′ | This study |
| phaC2-FOR | 5′-ATTCCAGATCAGGTCGTTGG-3′ | This study |
| phaC2-REV | 5′-GGTCAGCCTGCTGGATAGTC-3′ | This study |
| phaZ-FOR | 5′-CCTGCCCATAGTCGAGGTAA-3′ | This study |
| phaZ-REV | 5′-CTGGAGCTGGTGTTTCCATT-3′ | This study |
| phaC1-FOR | 5′-GCCAGGTAGGTTTGCAGGTA-3′ | This study |
| phaC1-REV | 5′-TCTGCTCGTATGGTGCTGAC-3′ | This study |