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. 2018 Dec 12;8:17778. doi: 10.1038/s41598-018-35463-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Baculovirus transduction into mosquito C6/36 cells. (a) Schematic representation of a composite expression vector for generating recombinant baculovirus. The composite transfer vector contains the sv40 and pag1 promoters, which are designed to express mCherry in mammalian and insect cells, respectively. (b) Dose-dependent entry of baculovirus. C6/36 cells were transduced with different concentrations (MOI = 1, 10, and 100) of recombinant vABspmC baculovirus. (c) Gating of mCherry-positive cells by flow cytometry, shown the efficiency of baculovirus transduction using different MOIs. (d) Quantification of mCherry-positive cells to indicate transduction efficiency. (e) GP64-mediated entry of baculovirus. C6/36 cells were treated with a baculovirus-antibody mixture (neutralizing or non-neutralizing antibodies against GP64 protein). The mCherry fluorescence images were taken at 48 h post-transduction in both panel’s (b and e). Data represent the average ± SD (standard deviation) of three biological replicates (n = 3).