Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 12;8:17778. doi: 10.1038/s41598-018-35463-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of baculovirus transduction on cellular proliferation and toxicity. (a) Schematic diagram showing the experimental design. (b) Flow cytometry analysis of cells labeled with CPD eFluor450 dye. Representative dot plots of mock, mock + CPD eFluor450 and vABspmC + CPD eFluor450 (MOI = 1) for 24 h were shown as an example for gating of populations. (c) Representative histograms of CPD eFluor450 labeling intensity in baculovirus transduced cells (Q2 population only) and MOCK cell (Q3 population only) at the indicated time points. (d) Analysis of mean CPD eFluor450 labeling intensity in mock and baculovirus transduced cells with different MOIs at various time points. (e) Cell viability. Toxicity of baculovirus transduction into C6/36 cells at varying dosages, measured every 4 days by AlamarBlue cell viability assay. (f) Persistent baculovirus-mediated gene expression. C6/36 cells were transduced with baculovirus at MOI = 1 and expression of fluorescent protein was analyzed over several days. The mCherry fluorescence images at various time-points were taken by fluorescence microscopy. Data shown represent the averages of three biological replicates, with error bars showing standard deviations.