Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 12;8:17779. doi: 10.1038/s41598-018-36217-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Influence of miRNA-29b inhibitor on the XYLT1 mRNA expression level and XT activity in NHDF. NHDF cell lines (n = 2) were seeded and transfected with miRNA-29b inhibitor (miR-29b inh.) or a control miRNA (NC). The relative XYLT1 mRNA expression level was determined by quantitative real-time PCR after 48 h. Data were normalized to a normalization factor calculated from the geometric mean of HPRT, GAPDH and B2M mRNA expression levels. The values indicated are expressed as ratio to one cell line (a). The XT activity (b) was quantified after 48 and 144 h in cell culture supernatants by using a radiochemical enzymatic assay. The XT activity in dpm was referred to the DNA content of the appropriate cell lysate. Values are means +/− SEM for three biological replicates per cell line; *p < 0.05; ****p < 0.0001 (Mann-Whitney U test).