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. 2018 Dec 12;8:17779. doi: 10.1038/s41598-018-36217-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results of the electrophoretic mobility shift assay depicting Sp1 and Sp3 binding to Sp1 transcription factor binding sites identified in silico in the XYLT1 promoter region. Biotin-labeled DNA probes corresponding to Sp1 transcription factor binding sites SP1.1 to SP1.7 were incubated with recombinant Sp1 (a) or Sp3 (b) protein and analyzed on two independent gels (separated by the black line). In each line 1 (NC), an unlabeled DNA probe was used to demonstrate specificity of DNA-protein binding. Sp1 or Sp3 binding to the appropriate DNA probes was relatively quantified by using ImageJ while only a comparison between samples on one gel was performed. The strongest band in (a) or (b) represents the strongest binding of DNA to protein and was defined as 100%. ns: unspecific binding. Full-length gels (with total free probe band) are presented in Supplementary Figs S1 and S2.