Table 2.
List of PCR systems for quantitative real-time PCR.
| Gene | Protein | 5′-3′-sequence | TOA [°C] |
|---|---|---|---|
| hB2M | B2M | TGTGCTCGCGCTACTCTCTCTT CGGATGGATGAAACCCAGACA |
63.0 |
| hCOL1A1 | COL1A1 | GATGTGCCACTCTGACT GGGTTCTTGCTGATG |
63.0 |
| hGAPDH | GAPDH | AGGTCGGAGTCAACGGAT TCCTGGAAGATGGTGATG |
63.0 |
| hHPRT | HPRT | GCTGACCTGCTGGATTAC TGCGACCTTGACCATCTT |
63.0 |
| hSP1 | Sp1 | GAGCTACAGAGGCACAAACG CCTGGGCCTCCCTTCTTATTC |
63.0 |
| hXYLT1 | XT-I | GAAGCCGTGGTGAATCAG CGGTCAGCAAGGAAGTAG |
63.0 |
| hXYLT2 | XT-II | ACACAGATGACCCGCTTGTGG TTGGTGACCCGCAGGTTGTTG |
63.0 |