Fig. 1.

Acute low glucose increased AMPK pathway activation. Representative immunoblots from HPA (a; n = 8) and U373 astrocytoma cells (b; n = 3) exposed to 2.5 or 0.1 mmol/l glucose media for 30 min. (c, d) Densitometric analysis of immunostaining for pT172 AMPK normalised to actin as a loading control in HPA (c) and U373 astrocytoma cells (d). (e, f) Densitometric analysis of immunostaining for pS79 ACC normalised to actin as a loading control in HPA (e) and U373 astrocytoma cells (f). Error bars represent SEM. *p < 0.05, one-sample t test vs control (2.5 mmol/l glucose)