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. Author manuscript; available in PMC: 2018 Dec 12.
Published in final edited form as: ACS Biomater Sci Eng. 2018 Feb 3;4(3):900–918. doi: 10.1021/acsbiomaterials.7b00831

Figure 6.

Figure 6

PLGA MP uptake induces suppressive DCs, resulting in decreased allogeneic T cell proliferation downstream. Dendritic cells were pretreated with PLGA MPs for 48 h and then cocultured with allogenic CD4+ T cells for 72 h or challenged with LPS for 24 h then cocultured with allogenic CD4+ T cells for 72 h. Allogenic T cell proliferation was measured via CFSE dye dilution. Controls in this experiment included a population of T cells only and T cells exposed to a stimulatory cocktail of PMA and IONO. The * symbol represents a pairwise comparison to the DC + T cell group (p ≤ 0.05). The $ symbol represents a pairwise comparison to the DC+ T cell+ LPS group (p ≤ 0.05). Data shown represent the mean ± standard error (n = 3 biological replicates).